Red Blood Cell Lysis Solution from Miltenyi Biotec

(1) Phenotyping EMT and MET cellular states in lung cancer patient liquid biopsies at a personalized level using mass cytometrySci RepDecember 8, 2023Loukia G. Karacosta, Danny Pancirer, Jordan S. Preiss, Jalen A. Benson, Winston Trope, Joseph B. Shrager, Arthur Wai Sung, Joel W. Neal, Sean C. Bendall, Heather Wakelee, et al.PEs were bloody, red blood cells were removed using the Red Blood Cell Lysis Solution ((Miltenyi, #130-094-183). Following red blood cell lysis, samples were washed with RPMI media and centrifuged at 300×g for 7

(2) Dietary Supplementation with Nucleotides, Short-Chain Fructooligosaccharides, Xylooligosaccharides, Beta-Carotene and Vitamin E Influences Immune Function in KittensAnimals (Basel)December 2, 2023Jujhar Atwal, Willy Joly, Robyn Bednall, Fabio Albanese, Michelle Farquhar, Lucy J. Holcombe, Phillip Watson, Matthew Harrisontotal of 100 µL of lysis buffer (red cell lysis buffer (Miltenyi biotec, Bergisch Gladbach, Germany, 130-094-183) diluted 1:2 with distilled water (Life Technologies, Carlsbad, CA, USA, 15230162) plus 0.3 µL/mL of

(3) Expansion of CD4+ cytotoxic T lymphocytes with specific gene expression patterns may contribute to suppression of tumor immunity in oral squamous cell carcinoma: single-cell analysis and in vitro experimentsFrontiers in ImmunologyNovember 23, 2023Hu Chen, Junsei Sameshima, Shiho Yokomizo, Tomoki Sueyoshi, Haruki Nagano, Yuka Miyahara, Taiki Sakamoto, Shinsuke Fujii, Tamotsu Kiyoshima, Thomas Guy, et al.EasySep Buffer (20144, STEMCELL), erythrocytes were eliminated using a Red Blood Cell Lysis Solution (130-094-183, Miltenyi Biotec). For CD45+ cell isolation, the homogenized cell suspension was incubated with a PE anti-human CD45

(4) Liver cancer stem cell dissemination and metastasis: uncovering the role of NRCAM in hepatocellular carcinomaJournal of Experimental & Clinical Cancer ResearchNovember 22, 2023Lingyun Zhou, Linye He, Chang-Hai Liu, Huandi Qiu, Li Zheng, Klarke Michael Sample, Qin Wu, Jiaxin Li, Kunlin Xie, Javier Ampuero, et al.fluorescence Cell Analyzer (Countstar Rigel S2) with AO/PI reagent after the removal of erythrocytes (Miltenyi 130–094-183), debris (Miltenyi 130–109-398) and dead cells (Miltenyi 130–090-101). Finally, fresh cells were washed

(5) Multiomic analysis reveals cellular and epigenetic plasticity in intestinal pouches of ulcerative colitis patientsmedRxivNovember 17, 2023Yu Zhao, Ran Zhou, Bingqing Xie, Cambrian Y Liu, Martin Kalski, Candace M Cham, Jason Koval, Christopher R Weber, David T Rubin, Mitch Sogin, et al.10% FBS [Gemini Bio 100–106]). Cell suspension was then diluted by 2 ml lysis buffer [Miltenyi Biotec 130–094-183] and sat for 3 minutes at room temperature to eliminate red blood cells. Cells in suspensions were spun

(6) Single-cell RNA sequencing reveals cancer stem-like cells and dynamics in tumor microenvironment during cholangiocarcinoma progressionFrontiers in Cell and Developmental BiologyNovember 10, 2023Jihye L. Golino, Jing Bian, Xin Wang, Jianyang Fu, Xiao Bin Zhu, Julie Yeo, Michael Kelly, Freddy E. Escorcia, Maggie Cam, Changqing Xiefor sequencing. In between, the samples were treated with Red Blood Cell Lysis Solution (Miltentyi # 130-094-183) and Debris Removal Solution (Miltenyi Biotec 130-109-398). The cells were maintained on ice during

(7) Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin KScientific ReportsNovember 7, 2023Yuka Miyahara, Hu Chen, Masafumi Moriyama, Keita Mochizuki, Naoki Kaneko, A. S. M. Rafiul Haque, Akira Chinju, Kazuki Kai, Mizuki Sakamoto, Noriko Kakizoe-Ishiguro, et al.using its Cell-Strainer Cap (Catalog No. 352235; FALCON) and Red Blood Cell Lysis Solution (Catalog No. 130-094-183; Miltenyi Biotec) was used in the lysis of erythrocytes. After these steps, the collected cells were

(8) A single‐cell transcriptomic atlas characterizes age‐related changes of murine cranial stem cell nichesAging CellNovember 1, 2023Bo Li, Jingya Li, Bingzhi Li, Takehito Ouchi, Longjiang Li, Yu Li, Zhihe Zhaoin 100 μL 1× PBS (0.04% BSA) and added with 1 mL 1× Red Blood Cell Lysis Solution (Miltenyi Biotec, 130‐094‐183) and incubated on ice for 5–10 min. After incubation, the suspension was centrifuged at 420 g for

(9) Intravenous BCG vaccination reduces SARS-CoV-2 severity and promotes extensive reprogramming of lung immune cellsiScienceOctober 20, 2023Singh AK, Wang R, Lombardo KA, Praharaj M, Bullen CK, Um P, Gupta M, Srikrishna G, Davis S, Komm O, et al.BSA and pelleted at 4°C. The RBC lysis was carried out using 1X RBC lysis solution (Miltenyi Biotec; 130-094-183) in a total volume of 1ml for 10 minutes at 4°C. Cells were resuspended in 10 ml chilled DPBS containing

(10) TAZ facilitates breast tumor growth by promoting an immune‐suppressive tumor microenvironmentMolecular OncologyOctober 16, 2023Anat Gershoni, Ori Hassin, Nishanth Belugali Nataraj, Sivan Baruch, Adi Avioz‐Seligman, Anna Chiara Pirona, Liat Fellus‐Alyagor, Tomer Meir Salame, Saptaparna Mukherjee, Giuseppe Mallel, et al.were filtered through 70 μm meshes to remove non‐dissociated clumps. Red blood cell lysis solution (#130‐094‐183, Miltenyi) was used to remove red blood cells; the solution was applied to the filters, incubated with

(11) Spatially multicellular variability of intervertebral disc degeneration by comparative single-cell analysisCell proliferationOctober 1, 2023Lin P, Yan P, Zhu J, Huang S, Wang Z, Hu O, Jin H, Li Y, Zhang L, Zhao J, et al.tissue debris. The single cell suspension was then treated with the 1× red blood cell lysis solution (130–094‐183, Miltenyi Biotec) to remove the residual blood cells. We eliminated dead cells by labelling cells with

(12) Spinal cord injury dysregulates fibro-adipogenic progenitors miRNAs signaling to promote neurogenic heterotopic ossificationsCommunications BiologySeptember 12, 2023Jules Gueguen, Dorothée Girard, Bastien Rival, Juliette Fernandez, Marie-Emmanuelle Goriot, Sébastien Banzetfiltered through a 70 µm cell strainer. Red blood cell lysis was performed using commercial buffer (130-094-183, Miltenyi). Cells were incubated in FcBlock (130-092-575, Miltenyi) for 30 min and control isotypes

(13) CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effectsScientific reportsSeptember 1, 2023Dhumale P, Nielsen JV, Hansen ACS, Burton M, Beck HC, Jørgensen MG, Toyserkani NM, Haahr MK, Hansen ST, Lund L, et al.Initially, the isolated ADRC was subjected to red cell lysis (RBC buffer, Miltenyi Biotech, cat no.130-094-183). Magnetic labelling was performed using a CD31 microbead kit (Miltenyi Biotech) after which cells were

(14) Maf1 is an intrinsic suppressor against spontaneous neural repair and functional recovery after ischemic strokeJournal of advanced researchSeptember 1, 2023Tsang CK, Mi Q, Su G, Hwa Lee G, Xie X, D'Arcangelo G, Huang L, Steven Zheng XF.suspension was gone through the red blood cell removal step using the Red Blood Cell Lysis Solution (#130-094-183), and then Myelin Removal Beads II (Miltenyi Biotec catalog #130-096-731) was used for clearance of

(15) Natural killer cell activation by Ubiquitin-specific protease 6 (USP6) mediates tumor suppression in Ewing sarcomaCancer Research CommunicationsAugust 22, 2023Kanika Jain, Ian C. Henrich, Laura Quick, Robert Young, Shreya Mondal, Andre M. Oliveira, Gerd A. Blobel, Margaret M. Chou(EDTA)-coated tubes, and red blood cells (RBC) were lysed using RBC lysis buffer (Miltenyi Biotec #130-094-183). The remaining white pellet (leukocytes) was subjected to flow cytometry analysis to quantify NK cells

(16) G9a inactivation in progenitor cells with an Isl1-Cre with reduced recombinase activity models aspects of Dandy-Walker complexBiology OpenAugust 15, 2023Lijun Chi, Ling Zhong, Dorothy Lee, Xinwen Yu, Amalia Caballero, Brian Nieman, Paul Delgado-Olguinthe resulting pellets were then incubated in 1× Red Blood Cell Lysis Solution (MACS Miltenyi Biotec, 130-094-183) at room temperature for 10 min. Subsequently, the cells were resuspended in 250 µl Dulbecco's modified

(17) TRIM28 modulates nuclear receptor signaling to regulate uterine functionNat CommunAugust 1, 2023Rong Li, Tianyuan Wang, Ryan M. Marquardt, John P. Lydon, San-Pin Wu, Francesco J. DeMayoµm and 70 µm mesh sequentially. The blood cells and debris were removed by red blood cell lysis kit (130-094-183, Miltenyi Biotec). ATAC-seq, RNA-seq, ChIP-seq and scRNA-seq analysis The fastq files were trimmed,

(18) Exploring the cellular and molecular differences between ovarian clear cell carcinoma and high-grade serous carcinoma using single-cell RNA sequencing and GEO gene expression signaturesCell & BioscienceJuly 31, 2023Dan Guo, Sumei Zhang, Yike Gao, Jinghua Shi, Xiaoxi Wang, Zixin Zhang, Yaran Zhang, Yuming Wang, Kun Zhao, Mei Li, et al.centrifugation at 400 × g for 10 min. Red blood cells were lysed with RBC lysis buffer (Miltenyi Biotec, 130-094-183) for 2 min on ice, and dead cells were removed with a dead cell removal kit (Miltenyi Biotec, 130-090-101)

(19) Phosphorylated nuclear DICER1 promotes open chromatin state and lineage plasticity of AT2 tumor cells in lung adenocarcinomasScience AdvancesJuly 28, 2023Raisa A. Reyes-Castro, Shin-Yu Chen, Jacob Seemann, Samrat T. Kundu, Don L. Gibbons, Swathi Arurtransferred to a 2-ml tube. Red blood cells were lysed using red blood cells lysis buffer (Miltenyi Biotec, 130-094-183), and the lysate was centrifuged for 1 min at 5000 rpm. Supernatant was removed, and cells in the pellet

(20) Identification and Characterization of the Wilms Tumor Cancer Stem CellAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2023Petrosyan A, Villani V, Aguiari P, Thornton ME, Wang Y, Rajewski A, Zhou S, Cravedi P, Grubbs BH, De Filippo RE, et al.rpm for 5 min and erythrocytes were eliminated using a red blood cell lysis kit (Miltenyi Biotec, #130‐094‐183). Aliquots of isolated cells were stored in CryoStor cell cryopreservation medium (Sigma‐Aldrich, #C2874)

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7/6/2016Good for Lysis of Red Blood Cells Jesus CirizaWhen working with blood samples this is a good solution to remove red blood cells

Red Blood Cell Lysis Solution (10x; 50 mL)

Red Blood Cell Lysis Solution from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Red Blood Cell Lysis Solution from Miltenyi Biotec

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